Background The oncogene a disintegrin and metalloproteinase 9 (ADAM9) was up\regulated in ovarian cancer tissues, and the present study aims to explore the potential diagnostic and prognostic value of ADAM9 in ovarian cancer (OC). in the non\tumorous tissue (61/90 vs 47/90), and increased expression level of D panthenol ADAM9 may associate with higher histological grade, advanced Figo stage and increased risk of metastasis; moreover, the mRNA expression of ADAM9 was also increased in OC tissue compared with the normal tissue (test was performed to for comparison between two groups. Correlation between the expression of the ADAM9 and the clinical characteristic of the OC patients was analyzed by chi\square test. Receiver operating characteristics (ROC) curve was drawn to evaluate the potential diagnostic value of ADAM9. Kaplan\Meier survival analysis was performed to compare the overall survival (OS) and disease\free survival (DFS) of the OC patients in the different groups. valuevalues /th th align=”left” valign=”bottom” rowspan=”1″ colspan=”1″ Unfavorable (n) /th th align=”left” valign=”bottom” rowspan=”1″ colspan=”1″ Positive (n) /th /thead Age (years)??? 50815.761 502146?Histological grade???Well\modulate1545.038* Poor1416?FIGO stage???I/II1647.034* III/IV1314?Metastasis???No1042.002** Yes1919? Open in a separate window * em P /em ? ?.05. ** em P /em ? ?.01. 3.2. Increased expression of ADAM9 in fresh OC tumor tissue samples and its potential diagnostic value Furthermore, the expressions of ADAM9 in 30 fresh OC tumor samples and the adjacent normal tissue were compared by RT\qPCR methods. We observed that this expression of ADAM9 was significantly increased in OC tissue compared with the normal tissue (Physique ?(Physique2A,2A, em P /em ? ?.001); moreover, results of ROC analysis suggested that this AUC of ADAM9 for OC was 0.8389 (Figure ?(Physique2B,2B, 95% confidence interval (CI) 0.7333 to 0.9445), indicating that ADAM9 is a D panthenol sensitive marker for the diagnosis of OC. Open in a separate window Physique 2 A disintegrin and metalloproteinase 9 (ADAM9) may serve as a diagnostic marker for ovarian cancer (OC). A, Comparison of the mRNA expression of ADAM9 between the OC tumor and the adjacent non\tumorous tissue by RT\qPCR methods. B, Results of ROC analysis. *** em P /em ? ?.001 3.3. Over\expression of ADAM9 may indicate poor prognosis of patients with OC Finally, we performed Kaplan\Meier survival analysis to investigate the roles of ADAM9 expression in the evaluation of OS and D panthenol DFS of the OC patients. As shown in Physique ?Physique3,3, both the OS (Physique ?(Physique3A,3A, em P /em ?=?.004) and the DFS (Physique ?(Physique3B,3B, em P /em ?=?.014) of OC patients with significantly lower in the ADAM9 positive group compared with the ADAM9 negative group, which suggested that increased ADAM9 may indicate poor prognosis of OC patients. Open in a separate window Physique 3 A disintegrin and metalloproteinase 9 (ADAM9) may serve as a prognostic marker for ovarian cancer (OC). A, Comparison of the OS of the ADAM9 positive and ADAM9 unfavorable OC patients. B, Comparison of the DFS of the ADAM9 positive and ADAM9 unfavorable OC patients 4.?DISCUSSION In the present study, we have explored the roles of ADAM9 in OC and its PIP5K1C clinical significance. We found that ADAM9 was significantly up\regulated in OC tissue compared with the normal tissue, and we also exhibited that AMAM9 may serve as D panthenol potential diagnostic and prognostic marker for the early diagnosis and treatment of OC. The roles of ADAM9 in different types of cancers have been discussed in many previous works. Caporali et al suggested that microRNA\126\3p may contribute to the dabrafenib resistance of melanoma via up\regulating ADAM9 Caporali16; Oria et al found that ADAM9 may contribute to the development of pancreatic ductal adenocarcinoma17; Dong et al exhibited that ADAM9 can induce the epithelial\mesenchymal transition of the hepatoma cells18; Wang et al suggested that ADAM9 functions as an oncogene in gastric cancer, and it was negatively regulated by micoRNA\126. 14 A study on the roles of ADAM9 in OC is limited. Ueno et al reported that ADAM9 is over\expressed in OC, and may contribute to the cisplatin sensitivity of OC cells.15 In the present study, we observed that the positive rate of ADAM9 in PFFE OC tissue was significantly increased in tumor tissue compared with the adjacent tissue, which was consistent with the Ueno et al’s observation; moreover, the expression of ADAM9 was positively associated with a higher histological grade, Figo stage, and metathesis of the tumor. Taken together, these data indicated that ADAM9 was up\regulated in OC and may function as an oncogene. The early diagnosis of OC is of great importance to increase the survival of the patients.19, 20, 21 It is still unclear.