The mice weighted about 272g (24.7C28.9g). in isolated mice islets for the respective pathways to elucidate their activities. Elevated concentrations of Acetylcholine and GLP-1 in treated mice were found to be sufficient to activate the respective pathways for insulin secretion (p<0.05). The amount of membrane bound GLUT1 and GLUT4 transporters were quantified and the subsequent glucose uptake and glycogen LRE1 synthesis were assayed. We showed that levels of membrane bound GLUT4 transporters, glucose-6-phosphate in skeletal myocytes, activity of glycogen synthase, and level of glycogen deposited in the skeletal muscles all increased (p<0.05). Conclusion Findings of the present study clearly prove the role of Acetylcholine and LRE1 GLP-1 in the Insulin secreting activity of subsp. Cicla, also known as Chard, is a leafy green vegetable, with a red stalk and is widely consumed throughout the world. It has been traditionally used as an anti-hyperglycemic nutritional supplement in Turkey[1]. Nutrition therapy is an important part of diabetes self-management education. The primary objective of diabetes Ik3-1 antibody self-management education has been to reduce the incidence of various diabetic complications, to minimize morbidity, and to reduce overall treatment cost[2]. However, concrete evidence regarding this is sparse, and misconceptions about this method of diabetes management, amongst patients, are rife[3]. Studies conducted by ? Bolken et al (2000), have shown an increase in the number of -cells in diabetic rats on B. vulgaris administration. Other significant observations made in this study were an increase in number of secretory granules, low density granules and hypertrophic Golgi bodies. Overall, the study predicted possible -cell regenerating capability of was demonstrated on diabetic rabbits as well[1]. Histological examinations, assay of serum urea, and creatinine of treated diabetic rats also showed significant nephro-protective activity of this plant extract[5]. Biochemical studies conducted on hepatic enzyme functions ascertained significant protection imparted upon the liver of diabetic rats[4]. Additionally, investigators LRE1 have shown that might help to retard the onset of various ocular complications, commonplace in diabetic patients[6]. also demonstrated to have a highly promising anti-oxidative effect in studies on diabetic rats. The study showed a reversal in the increased lipid peroxidation and lowered glutathione levels in the aorta and cardiac tissues of diabetic rats[7]. Other studies have corroborated this finding by investigating the anti-oxidant properties of this plant[8]. From this body of available literatures, we have taken LRE1 a particular desire for the works of ?Bolken et al (2000). The authors of this paper have concluded potential -cell regeneration behind the anti-hyperglycemic activity of in conjunction with the respective pharmacological inhibitors, enhancers and LRE1 antagonists of acetylcholine and GLP-1 to manipulate their plasma levels or to get rid of their effect. The resultant plasma insulin was observed after each treatment. We replicated the same in-vivo concentration of Acetylcholine and GLP-1 in-vitroand revealed the mice islets, isolated from pre-treated mice, to observe whether the concentration of Acetylcholine and GLP-1 observed in-vivo are capable of activating the insulin launch cascade in-vitro. The second part of this paper entailed observing the glucose uptake and determining fate of this glucose. Membrane bound GLUT-1, GLUT-4 transporters and glucose-6-phosphate were quantified. Hexokinase II activity and glycogen synthase activity were identified along with the glycogen deposited in the skeletal muscle tissue. Material and Methods 1. Flower Collection and Control (whole flower) was procured from University or college Ayurvedic Research Centre (UARC), Jahangirnagar University or college, Dhaka, Bangladesh. Identity of the flower material was confirmed by a botanist, and a voucher specimen was deposited in the National Herbarium at Mirpur, Dhaka, Bangladesh. The flower.