Objective To judge some agricultural waste materials extracts as insecticide and their effects in enzyme activities in liver organ and kidney of male mice. of Entomology Department-Faculty of Science-Ain Shams University where self-perpetuating colonies had been preserved and set up through the present research. Primary toxicological bioassay checks were carried out on tested compounds as a modification TH-302 for the method explained by Wright 1971. Mortality data was analyzed by using log-probity analysis to estimate probity regression collection and calculate LC50 LC90 slope function by applying the computer system of Unkelbach. 2.3 Toxicological evaluation of the preferred substances on experimental animals 2.3 Acute toxicity Sixty adult Wister TH-302 rats of both sexes for every compound bought from animal home colony in Country wide Research Middle Giza Egypt. The pets weighing about (200 ± 20) g had been split into six identical groups each filled with 10 pets. All rats had been kept under managed conditions of heat range (22 ± 1) °C and dampness 60% ± 5%. These were given pellet taking in and food water ad libitum. The experimental process met the nationwide direct lines on the correct care and usage of pets in the lab analysis. 2.3 LD50 perseverance To look for the lethal dose that eliminates half (50%) from the animals tested “LD50” for preferred compounds dark and white liquor 6 doses of every chemical substance ranged from 500 to 5 000 mg/Kg bodyweight were utilized. Each dosage from both substances was dissolved in 0.5 mL water and was implemented orally to rats using belly pipe with ball tipped oral incubated needle. The LD50 worth of both substances was computed for 24 h. mortalities pursuing each treatment. 2.3 Sub-acute toxicity Sets of Swiss albino mice of both sexes (8-10 weeks previous) weighing (22 ± 1) g had been housed under regular laboratory circumstances and had free of charge access to water and food. In research of sub-acute toxicity of white and dark liquor several 40 mice was found in each treatment for both substances. In several 30 mice were used seeing that control parallel. Doses equal to 4 mg/mice/time of every compound had been dissolved in 100 μL of drinking water and implemented orally for 14 days. At the same time another two sets of 40 mice for every group were utilized to evaluate the toxic aftereffect of organophosphorus and pyrethroid insecticides Fenitrothion and Deltamethrin against both previous TH-302 substances. Mice treated daily with Fenitrothion and Deltamethrin dissolved in dimethylsulphoxide (DMSO) at dosage 0.8 & 12.5 mg/mice/day matching to 1/20 LD50 respectively. Animals were noticed in regards to to behavior urge for food bodyweight gain as well as for signals of treatment-related effects during the feeding experiments. At the end of the experiment 2 weeks animals TH-302 were sacrificed under ether anesthesia and blood samples were taken from the orbital plexus of treated and control mice in heparinized tubes. Blood samples were centrifuged to separate plasma for different biochemical analysis. There are at least six animals in each control and treated group. 2.3 Bio-chemical analysis Acetyl cholinesterase (AChE) activity was assayed in blood samples using 0.1 M phosphate buffer. pH 7.2 and acetylthiocholine iodide while substrate according to the method of Ellman larvae in the laboratory. The results are offered in Table 1 and illustrated in Number 1. Table 1. Larval TNFSF13B mortality and LC50 & LC95 TH-302 ideals for the larvae exposed to some natural substances (west-product). Number 1. Susceptibility of larvae to some natural substances The confidential limits of each of the tested compound were statistically determined TH-302 for LC50 and LC95 at = 40). The activities of liver enzymes (AST ALT and ALP) are demonstrated in Table 3. During the whole experimental period 2 weeks no effect could be observed on the activities of these enzymes on mice treated with white and black liquor comparing to the control mice. Concerning the effect on kidney function Table 3 shows the concentration of urea and creatinine. There is no significant increase in the concentration of both above guidelines was observed within the treated mice when compared with control group with the end of the treated period. On contrast the level of alanine and aspartate aminotranferase enzymes (ALT &.