Third, in cells lacking UNC93B1, TLR9 isn’t detected within COPII vesicles
Third, in cells lacking UNC93B1, TLR9 isn’t detected within COPII vesicles. existence of EndoH-resistant glycans. Lysates had been separated by SDS-PAGE and immunoblotted using the indicated antibodies. EndoH-sensitive (white arrow) and EndoH-resistant (dark arrow) forms are indicated. (G) Mutation of UNC93B1 glycosylation sites abolishes EndoH resistant forms. Lysates from HEK293Ts transiently transfected with FLAG tagged … [Read more…]