Supplementary MaterialsSupplementary Details. and 50?for 10 minutes. Supernatant (free [125I]) and
Supplementary MaterialsSupplementary Details. and 50?for 10 minutes. Supernatant (free [125I]) and pellet (undamaged [125I]-Abinding to rPrPc, 96-well plates were coated with the indicated rPrPc concentrations in PBS starightaway at 4C on a shaker. Plates were used in 37C for thirty minutes and washed with ice-cold PBS twice. Unspecific binding sites had been obstructed with 3% … [Read more…]