2020;383(17):1694\1695. authors have positively contributed to the study. The study was designed by DB and TEJ. DS, SD, SB, CG, TM, KK, NR, AK, NS, AP, ST, and AEP, were involved in the data collection and overall performance of the study. DS and GN were involved in conducting the laboratory assessments. DB and TEJ did the data analysis. DB and TEJ prepared the manuscript. Final manuscript was go through and approved by all the authors. To the editors, The development and longevity of antibodies against SARS\CoV\2 in kidney transplant recipients (KTRs) is not obvious. Although seroconversion has been documented, 1 the persistence of this response with time is not established. We longitudinally analyzed the presence of anti\SARS\CoV\2 IgG antibodies in KTRs after symptomatic coronavirus disease\2019 (COVID\19). Forty\seven patients with a positive SARS\CoV\2 RTPCR between May 2020 and March 2021 were followed up in the transplant medical center for 5.55??2.32?months. Sera was initially tested at day 15 from diagnosis for the presence of anti\SARS\CoV\2 total IgG antibodies using COVID KAWACH IgG MICROLISA (J. Mitra Pvt. Ltd; approved by the Indian Council of Medical Research 2 ). It is a qualitative assay using SARS CoV\2 computer virus whole\cell antigen (sensitivity?=?96.33%, specificity?=?100%). Patients negative on day 15, were retested until they seroconverted. Nineteen of 47 (40.4%) patients developed severe COVID\19 as defined by Who Folic acid also (Table?S1). None of the patients developed reinfection. None of these patients were vaccinated against SARS\CoV\2 till data collection. Forty\one (87.2%) patients achieved seroconversion with majority (63.4%) achieving within 2?weeks (Physique?1A). Six (12.7%) patients who did not develop antibodies were repeatedly tested for 3.29??1.38?months (Physique?1B). Patients who seroconverted experienced higher body mass index ( em P /em ?=?.036) but there were no differences in the age, time post\transplant, baseline immunosuppression, graft function, severity of COVID\19, and immunosuppression modifications (Table?S1). Open in a separate window Physique 1 (A) Time to achieve initial seroconversion (n?=?41, remaining 6 patients did not achieve seroconversion). (B) Development of antibody response in recipients over time. (n?=?47). Patients with who failed to accomplish initial seroconversion were repeatedly tested over 1\6?months. For patients who switched negative with time, the test was not repeated any further, thus they are counted only once here Patients with positive IgG were repeatedly tested at an interval of 1\3?months till Folic acid the last follow\up (5.62??2.37?months). Seven of 41 (17.1%) patients became IgG negative with time (Physique?1B). With respect to persistence of antibody response, there was no difference associated with age ( em P /em ?=?.90), time post\transplantation ( em P /em ?=?.085), use of antibody induction ( em P /em ?=?.70), baseline antimetabolite use ( em P /em ?=?.99), and use of anti\rejection therapy in recent ( em P /em ?=?.99). 44.1% of patients with persistent seroconversion experienced a history of severe COVID\19 (vs 28.6% of those who become IgG negative, em P /em ?=?.68). Patients with prolonged antibody response were more likely to Folic acid have withdrawal of antimetabolite (71.9% vs 57.1% but insignificant em P /em ?=?.72) and reduction/withdrawal of calcineurin inhibitors (60% vs 42.9%, but insignificant em P /em ?=?.43) during COVID\19. None of the factors predicted prolonged immunological response in multivariate analysis. This study confirms that the majority of KTRs (87%) develop serological response after symptomatic COVID\19 and it is Folic acid sustained with time in 34/41 patients (83%). These results are in concurrence with Chavarot et al 3 who have reported seroconversion in 71.4% KTRs. However, in contrast, 60% of patients in their cohort, switched equivocal/unfavorable at 6?months. This can be attributed to the smaller severity of COVID\19 in their cohort (76% patients hospitalized) and higher age (57.7 years 4 ). Data from the general population suggest that antibody response is usually blunted in moderate COVID\19 and a decline can occur over 60\90 days. 4 , 5 In KTRs, this can be impacted by baseline immunosuppression, the severity of COVID\19, immunosuppression modifications during COVID\19. In our cohort, there is a transmission of prolonged seroconversion in patients with severe COVID\19, we could not establish statistical significance probably due to a ACVRL1 smaller sample size, which remains a limitation of this study. However, to our knowledge, this remains Folic acid the largest study evaluating immunological response in KTRs longitudinally and merits meticulous clinical correlation and follow\up. In conclusion, these data are reassuring about the development and persistence of seroconversion against SARS\CoV\2 in KTRs despite majority being on standard.