Supplementary MaterialsSupplementary Data. particular enrichment for poly(A) site junctions with no
Supplementary MaterialsSupplementary Data. particular enrichment for poly(A) site junctions with no need for complicated sample preparation, purification or fragmentation. Poly(A)-ClickSeq (PAC-seq) can be therefore a straightforward procedure that produces high-quality RNA-seq poly(A) libraries. Like a proof-of-principle, we used PAC-seq to explore the poly(A) panorama of both human being and cells in tradition and noticed exceptional … [Read more…]